Composite

Part:BBa_K1497007:Design

Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 02:43, 12 October 2014 by DSachs (Talk | contribs) (→‎Design Notes)

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Naringenin producing operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
    Illegal NheI site found at 4631
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1671
    Illegal BglII site found at 4640
    Illegal XhoI site found at 3638
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1777
    Illegal NgoMIV site found at 2609
    Illegal NgoMIV site found at 4611
    Illegal NgoMIV site found at 5187
    Illegal AgeI site found at 1872
    Illegal AgeI site found at 2038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3067
    Illegal BsaI site found at 4560
    Illegal BsaI.rc site found at 1303
    Illegal BsaI.rc site found at 4042


Design Notes

The functionality of this construct is verified by putting the device under a promotor. Proofing functionality leaded to Bba_K1497008 (Naringenin Operon under the constitutive promotor J23100) and Bba_K1497008 (Naringenin Operon under the T7 Promotor).

Source

References