Coding
PfrI

Part:BBa_K1493200

Designed by: Wen Wu   Group: iGEM14_Wageningen_UR   (2014-10-06)
Revision as of 09:12, 11 October 2014 by Wenchies (Talk | contribs) (→‎Characterization)

Ferric regulator Pfri

Pfri was obtained from Pseudomonas putida KT2440, it functions as a transcription regulator that initiates transcription of genes involved in pyoverdine synthesis. Pyoverdine is a flourescent compound that is produced by P.putida that binds to iron (III). This gene was used in order to try to increase pyoverdine production P.putida Read more on our wiki [http://2014.igem.org/Team:Wageningen_UR]


Cloning

Primers used for cloning were:
Fw: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCGGAACAACTATCCACAAGTAAG-3'
Rev: 5-'GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCAGGCCTGGCGACTGGC-3'
note: These primers already include the biobrick preffix and suffix


Charecterization

Characterization

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None