Generator

Part:BBa_K1481004

Designed by: Przemysław Nuc, Melania Nowicka, Weronika Krzynowek, Jakub Bartoszewicz, Katarzyna Rżosińska   Group: iGEM14_PoznanBioInf   (2014-10-10)
Revision as of 20:29, 10 October 2014 by Jakub (Talk | contribs)

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sfGFP with MLRR tag under arabinose promoter

This part generates a monomeric red fluorescent protein with his-tag for easy protein purification and an MLRR degradation tag. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for repression of pBAD in absence of arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1260


[edit]
Categories
Parameters
None