Generator
Bgal alpha

Part:BBa_K1477014:Experience

Designed by: 2014 Ivy Tech South Bend IN team   Group: iGEM14_IvyTech_SouthBend_IN   (2014-08-21)
Revision as of 16:16, 10 October 2014 by Jcampbell188 (Talk | contribs) (→‎Applications of BBa_K1477014)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1477014

"http://i.imgur.com/FwXzsYX.jpg

According to the Biorad 500 basepair DNA ladder that we used , K1477014 measures at ~2700 base pairs with a pSB1C3 backbone. This matches our quantitative data.

http://i.imgur.com/kwgTE7u.png

We also performed blue/white screening on x-gal plates. When the colonies grew they turned blue, proving that there is Beta-galactosidase activity occurring and that our inserted part is producing the alpha fragment to compliment the omega fragment which naturally occurs in Top 10 cells.

User Reviews

UNIQ2f3b65a6f31eb8b0-partinfo-00000000-QINU UNIQ2f3b65a6f31eb8b0-partinfo-00000001-QINU