Coding
T7 Immedia

Part:BBa_K1477030:Experience

Designed by: 2014 Ivy Tech South Bend IN team   Group: iGEM14_IvyTech_SouthBend_IN   (2014-08-22)
Revision as of 17:28, 2 November 2014 by Geotwaddle (Talk | contribs)

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Characterization and Applications of BBa_K1477030

Electrophoresis K1477030.png

In correspondence with a 500 basepair DNA ladder, our part measures at ~2500 base pairs which corresponds with the sum of the BioBricks and the pSB1C3 backbone. Plasmid DNA isolated from two subclones

Coliphage lysis of BBa K1477030.png

TEST FOR IMMEDIATE LYSIS OF TOP10 CELLS TRANSFORMED WITH BBa_K1477030. This immediate-lysis device was designed to be an immediate lysis cassette for T7 infection of transformed Top10 cells. The expected result was that a culture of transformed cells would be resistant to infection by T7 phage but not by an unrelated phage. This experiment represents transformed cells infected at a ratio of 100 cells to 1 pfu T7 phage. If functioning, the immediate lysis cassette should have stopped the propagation of T7 infection. What may be lacking is the presence of holin that increase the effectiveness of the endolysin.

User Reviews

UNIQ6669f3b5296b99a0-partinfo-00000000-QINU UNIQ6669f3b5296b99a0-partinfo-00000001-QINU