Coding

Part:BBa_K1484101

Designed by: Salil Bhate   Group: iGEM14_Cambridge-JIC   (2014-10-08)
Revision as of 11:32, 10 October 2014 by Salil (Talk | contribs)

GAL4-VP16 transcription activator CDS, compatible with PlantSyntax (RFC105)

GAL4 transcription activator. Binds to the UAS (upstream activation site) for activation of reporter expression in eukaryotes.

This part has been codon optimized for plant expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 226
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 695
    Illegal SapI site found at 206


[edit]
Categories
//chassis/eukaryote
Parameters
None