Coding

Part:BBa_K1420002

Designed by: Stephen C. Heinsch   Group: iGEM14_Minnesota   (2014-10-09)
Revision as of 01:52, 10 October 2014 by Valeriubortnov (Talk | contribs)

merB, Organomercurial Lyase from Serratia marcescens

Function

The merB gene is often found immediately downstream of merA, and is essential for the detoxification and bioremediation of organic toxic mercury compounds in congruence with merA. The merB protein is a lyase that catalyzes the breaking of carbon-mercury bonds through protonolysis of toxic mercury compounds, such as methylmercury (Scheme 1). This produces the less toxic and less mobile Hg2+ which is then completely volatilized to Hg0 when acted upon the enzyme merA.

Scheme 1. MerB Catalyzed Reaction (R= alkyl, aryl)

MerB reaction1.JPG

Structure and Mechanism

Enzyme MerB forms a dimer by a 2-fold pseudosymmetry around the depicted rotation axis in Figure 1. The two subunits are color coded from blue to green distinguishing the amino-terminal end of the protein from the carboxy-terminal, respectively. Active site residues are color coded as well using van der Waals sphere representation: residues Cys-96 and Cys-159 are in yellow, and residue Asp-99 is in red. The structure of mercury-bound MerB and free MerB is extremely similar with the exception of the mercury ion. The mercury is bound to MerB by two sulfurs from the Cys-96 and Cys-159. An oxygen from a water molecule is also involved, binding to the mercury atom. On the other hand, the active site residues for the free MerB are completely inaccessible.

MerB can catalyze the protonolysis of carbon mercury bonds for a number of organomercurials, accelerating the rate of this reaction up to 107 times the rate of spontaneous abiotic decay. Two proposed mechanisms for how this is accomplished is displayed in Figure 2. Both mechanisms assume that the methylmercury substrate forms an initial bond with Cys96 and that the Cys159 site is protonated. In Mechanism 1, Cys159 protonates the methyl carbon and forms a covalent bond with Hg(II). In Mechanism 2 however, Cys159 donates a proton to Asp99 before forming a bond with Hg(II). Asp99 is then responsible for protonating the methyl group.

MerB structure.JPG

MerB mechanism2.JPG

Figure 2. Proposed mechanisms of MerB catalysis for the Hg-C protonolysis reaction.

merB Experimental Results

The function of merB in the mer operon for the conversion of methylmercury to Mg(II) was tested in E. coli K12 strain cells which is depicted in Figure 3. For line that represents the bioremediation of methylmercury in abiotic environments, methylmercury is converted at an extremely low rate. The rate of methylmercury conversion when encapsulation beads were involved matched the rate corresponding to E. coli K12 strain containing the pBBRBB::gfp control strain, which is still seen to take a considerable amount of time (well over 25 hours). The E. coli K12 containing the pBBRBB::mer, however, is seen to remove methylmercury from the system almost completely after on 8 hours.


References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds
Parameters
None