Coding

Part:BBa_K1420002

Designed by: Stephen C. Heinsch   Group: iGEM14_Minnesota   (2014-10-09)
Revision as of 23:59, 9 October 2014 by Heins049 (Talk | contribs)

merB, Organomercurial Lyase from Serratia marcescens

Function

The merB gene is often found immediately downstream of merA, and is essential for the detoxification and bioremediation of organic toxic mercury compounds in congruence with merA. The merB protein is a lyase that catalyzes the breaking of carbon-mercury bonds through protonolysis of toxic mercury compounds, such as methylmercury. This produces the less toxic and less mobile Hg2+ which is then completely volatilized to Hg0 when acted upon the enzyme merA.

Structure

Mechanism File:Https://static.igem.org/mediawiki/2014/2/2f/MerB mechanism.PNG

Experimental Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds
Parameters
None