Composite

Part:BBa_K1446003:Experience

Designed by: Philipp Lorenz   Group: iGEM14_Oxford   (2014-09-29)
Revision as of 22:11, 9 October 2014 by Francescadonnellan (Talk | contribs) (→‎Applications of BBa_K1446003)

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Applications of BBa_K1446003

We cloned this part with an mCherry fusion at the C terminus of the dcmR gene into......

The changing mCherry fluorescence shows that the dcmR gene was expressed under the control of the tet promoter and operator system. Expression was induced with various amounts of ATC and the following fluorescence data acquired. Exposure time was 0.2 seconds. As no calibration data was obtained using purified mCherry, the results have been left in fluorescence arbitrary units.

Oxford DcmR-mCherry expression induced by 0ng ATC.png
Oxford DcmR-mCherry expression induced by 10ng ATC.png
Oxford DcmR-mCherry expression induced by 50ng ATC.png
Oxford DcmR-mCherry expression induced by 100ng ATC.png
Oxford DcmR-mCherry expression induced by 200ng ATC.png
Oxford Comparison of mean flouresence intensity from DcmR-mCherry expression induced at varying amounts of ATC.png

User Reviews

UNIQ31b0a7e9b211ba3b-partinfo-00000000-QINU UNIQ31b0a7e9b211ba3b-partinfo-00000001-QINU