Plasmid

Part:BBa_K1321302

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 11:42, 10 October 2014 by Mf1512 (Talk | contribs)

pSEVA331-BB with J23100-RFP

This part contains Anderson promoter J23117 and RFP coding device in pSEVA331-BB backbone. pSEVA331-BB is a broad host range plasmid converted into biobrick format, and is capable of replication in E.coli and several other G- bacterial species. We have verified that it also replicates in the cellulose producing bacterium Gluconacetobacter xylinus. We have used this part to express RFP in G. xylinus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3776
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3776
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
    Illegal NotI site found at 913
    Illegal NotI site found at 3782
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3776
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3776
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3776
    Illegal XbaI site found at 3791
    Illegal SpeI site found at 37
    Illegal PstI site found at 920
    Illegal NgoMIV site found at 2095
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None