Part:BBa_K1321116
Linker-cipA-linker + phytochelatin (PC) EC20 with LacI promoter
A LacI-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: 
Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 383
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 383
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 383
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 383
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 383
Illegal NgoMIV site found at 230 - 1000COMPATIBLE WITH RFC[1000]
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