Part:BBa_J63009:Design

Low copy protein fusion vector (Silver lab standard)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2057
  Illegal XbaI site found at 2072
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2057
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  Illegal NotI site found at 8
  Illegal NotI site found at 2063
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2057
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Illegal EcoRI site found at 2057
  Illegal XbaI site found at 2072
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2057
  Illegal XbaI site found at 2072
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1096
  

Design Notes

If the part starts with the nucleotides tc, then that part-containing plasmid must be grow in a DamI- strain to avoid methylation (and inhibition of digestion) at the XbaI site.

from [http://openwetware.org/wiki/Silver:_Commonly_Used_Plasmids Silver: Commonly Used Plasmids] on OpenWetWare:

• "We commonly use two Biobrick vectors: V0120 (same as V0100) and V0002. The V0120 vector preferred over the V0002 vector because V0120 is a high-copy plasmid (~300 ng/uL from a mini-prep of a 5 mL culture) while V0002 is not. The V0002 plasmid is most frequently found in older parts."

• "The annonated sequence of V0002 can be found [http://openwetware.org/images/5/5c/BBa_V0120.gb here]"

Source

based on pUC19 plasmid