Coding
Part:BBa_K1321364:Design
Designed by: Chris N Micklem Group: iGEM14_Imperial (2014-10-08)
NiBP fused to dCBD with linker driven by T7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 53
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 232
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
The promotor was cloned onto the protein via the XbaI/SpeI sites.
Source
asdf