Coding
Part:BBa_K1321360:Design
Designed by: Chris N Micklem Group: iGEM14_Imperial (2014-10-08)
CBDclos fused to sfGFP driven by LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 551
Design Notes
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Source
The LacI part came from BBa_J04500.
CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111.
Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.