Composite

Part:BBa_K1362054:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)
Revision as of 13:03, 17 October 2014 by Jelisaweta (Talk | contribs)

SsrA degradation tag as RFC[105] C-terminal insert


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 67


Design Notes

Source

C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli. The DNA sequence of the SsrA tag with overhangs was ordered as a DNA oligo and cloned into (BBa_K1362052).


References