Translational_Unit

Part:BBa_K1321335:Experience

Designed by: Laura de Arroyo Garcia   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 07:18, 23 October 2014 by Ld1411 (Talk | contribs) (→‎Applications of BBa_K1321335)


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Applications of BBa_K1321335

Part BBa_K1321335 was digested with XbaI and PstI and the resulting fragment, containing the optimised coding sequences of AcsC and AcsD, was cloned into a medium-to-low copy number plasmid (pSB3K3) containing the inducible pLAC promoter. The resulting construct, verified by GreenTaq Colony PCR, was subsequently sub-cloned into BBa_K1321336-containing electrocompetent cells. For more information on the characterisation procedure, click here: https://parts.igem.org/Part:BBa_K1321334:Experience

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