Coding

Part:BBa_K1321091:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-07)
Revision as of 17:47, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Design Notes)


Phytochelatin (PC) EC20 fused to CBDclos


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.

Source

PhytoC+CBD4. TBC

References