Composite
Part:BBa_K1371040:Design
Designed by: Yiran Wu Group: iGEM14_SCUT-China (2014-10-07)
Revision as of 05:32, 25 October 2014 by Invly leung (Talk | contribs)
DEBS1(KR1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1876
Illegal BamHI site found at 6423 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 556
Illegal NgoMIV site found at 2039
Illegal NgoMIV site found at 2411
Illegal NgoMIV site found at 2444
Illegal NgoMIV site found at 2917
Illegal NgoMIV site found at 3626
Illegal NgoMIV site found at 4499
Illegal AgeI site found at 367
Illegal AgeI site found at 1729
Illegal AgeI site found at 2075
Illegal AgeI site found at 2654
Illegal AgeI site found at 4375
Illegal AgeI site found at 4445
Illegal AgeI site found at 5072 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1481
Illegal BsaI.rc site found at 3911
Illegal SapI.rc site found at 2746
Design Notes
Candidate selection
Biosynthesis of polyketides like erythromycin, tacrolimus and many others are guided by modular PKS genes. These genes encode large enzymes consisting of modules of domains, forming an "assembly line" to extend the ketide units to form polyketides. Among the known PKS genes, the genes coding for erythromycin producing polyketide synthethase have been extensively and deeply studied, and there are numerous information sources. Therefore, we chose a truncated erythromycin-producing polyketide synthase which has been proved to produce a simple triketide lactone 1B.[2]
Source
Saccharopolyspora erythraea