Composite
Part:BBa_K1405004
Designed by: Xuan Wang, Shu Zhang Group: iGEM14_BNU-China (2014-10-07)
McfR
Background
McfR is an organic acid responsive chemoreceptor originally found in Pseudomonas putida. BBa_K1405004 contains an insulator, RBS sequence, and McfR coding sequence and its expression is under the control of the constitutive promoter BBa_J23100. To be continued.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1274
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/receptor
chassis | E.coli BL21 |
device_type | Chemotaxis receptor |
family | McpS |
ligands | malate, succinate |
origin | P. putida |
resistance | Chloramphenicol |