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Part:BBa_K1497023:Experience

Designed by: Sascha Hein, Sebastian Barthel, Thomas Dohmen, Sven Jager   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 22:58, 17 October 2014 by SaHein (Talk | contribs) (→‎Applications of BBa_K1497023)


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Applications of BBa_K1497023

For improving the enzyme the iGEM team TU Darmstadt 2014 decided to remove the tail and to construct their pelargonidin operon with this engineered ANS (eANS). The laboratory results cover the previous modeling results. The engineered ANS exhibited better yields than the original one when used in an operon producing pelargonidin (BBa_K1497015).



Figure 3 E.coli BL21 (DE3) pellet containing the pelargonidin producing operon after the fermentation. According to Yan et al. (2007) a pelargonidin producing E.coli should be red after a pelargenidin production. The operon with the engineered anthocyanindin synthase produces more pelargonidin

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