Protein_Domain
mlr A

Part:BBa_K1432001:Experience

Designed by: Chaohui Gao   Group: iGEM14_Jilin_China   (2014-10-06)
Revision as of 21:55, 2 November 2014 by ChaohuiGao (Talk | contribs) (→‎Applications of BBa_K1432001)

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Applications of BBa_K1432001

We will construct a mlr promoter-GPF-mlrA cluster and clone it into the E. coli-L. lactis shuttle expression vector. So it can be easily constructed in the laboratory and could avoid secondary pollution in the natural environment of E.coli.

we synthesized mlrA gene, there are 336 codons in Sphingomonas species ACM-3962. We replace 246 of them in order to increase the expression of mlrA because they are not abundant codons in Lactococcus lactis. And we successfully did it both in E.coli and L.lactis. When we induced the expression of protein, it shows that L.lactis could be more efficiently expressed than E.coli.

User Reviews

UNIQ409cf521d8cf8987-partinfo-00000000-QINU UNIQ409cf521d8cf8987-partinfo-00000001-QINU