Regulatory

Part:BBa_K1400004:Experience

Designed by: Dylan Siriwardena   Group: iGEM14_uOttawa   (2014-10-06)
Revision as of 21:29, 18 September 2015 by Arthanzel (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1400004

uOttawa 2015

The original application for this promoter was to use it to implement a tri-stable switch, which is thought to be involved in stem cell differentiation. However, when we were analyzing our models more in-depth, we found that the dynamics of this promoter do not lead to tri-stability.

Specifically, this promoter behaves multiplicatively. This means that adding a fixed amount of repressor will down-regulate the gene based on how much it is induced. For instance, adding a certain amount of repressor will cut the promoter's expression in half, rather than lowering it by a fixed amount.

The promoter also seems to behave differently when doxycycline is used instead of aTc to modulate repression by rtTA. With doxycycline, repression seems to be much weaker and activation with beta-estradiol modulating GEV seems to have a greater effect on the activity of the promoter.

User Reviews

UNIQ48201fcc41edd3f3-partinfo-00000000-QINU UNIQ48201fcc41edd3f3-partinfo-00000001-QINU