Measurement

Part:BBa_K1403000

Designed by: Urszula Czerwinska   Group: iGEM14_Paris_Bettencourt   (2014-10-03)
Revision as of 15:54, 6 October 2014 by Ula (Talk | contribs)

GFP generator in pSB1C3

Measurment kit test of Part:BBa_J23101 in pSB1C3. This part is a tween of BBa_I20260 but in a high copy pSB1C3 plasmid.

Usage and Biology

This construct produce GFP at high levels as it is placed in a high copy plasmid and the J23101 Anderson's promoter is a strong promoter. </br>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


[edit]
Categories
Parameters
None