Part:BBa_K1510011:Design

Extracellular endolysin

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 159
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The endolysin sequence from phage M102 contains a Spe1 cutting site, therefore, we design primers to make point mutation of the sequence in order to produce the same amino acid without the risk of being cut during circuit construction.

Source

J23100 is a strong constitutive promoter from 2014 igem distribution. The coding region, yebF and endolysin come from different origin. YebF is from E.coli K12, while endolysin is from the 19th opening reading frame of Streptococcus Mutans phage M102. Finally, B0015 is a double terminator from igem distribution.