Coding
Part:BBa_K1403019:Design
Designed by: Paris Bettencourt 2014 Group: iGEM14_Paris_Bettencourt (2014-10-06)
Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was submitted downstream a standard T7 constitutive promoter inside a pETDuet vector.
The ilvBN sequence was isolated from MG1655 through PCR and digested with NCoI and EcoRI to put into pETDUET1 vector. It was transformed into E.Coli strain with plasmid containing aldB to produce acetoin, which has the smell of butter.
Source
E. coli MG1655
References
David R. Nielsen, Sang-Hwal Yoon, Clara J. Yuan and Kristala L. J. Prather, Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli, January 2010.