Plasmid_Backbone

Part:BBa_K1465401:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-05)
Revision as of 16:26, 20 October 2014 by Tore (Talk | contribs)

Antibiotic-free selection plasmid (pSB1C3_alr_RFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3142
    Illegal NheI site found at 1893
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3148
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3142
    Illegal BglII site found at 1969
    Illegal BamHI site found at 1267
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 3018
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3142
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3142
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3157
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 1551
    Illegal AgeI site found at 1851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2094


Design Notes

AB_alr_fwd (66 bp) 5'-GAGTGGCAGGGCGGGGCGTAATTTTACTAGAAAGAGGAGAAACAAATGCAAGCGGCAACTGTTGTG-3'

AB_alr_rev (44 bp) 5'-CTATCAACAGGAGTCCAAGCGAGCTTAATCCACGTATTTCATCG-3'

pSB1C3_AB_fwd (66 bp) 5'-CACAACAGTTGCCGCTTGCATTTGTTTCTCCTCTTTCTAGTAAAATTACGCCCCGCCCTGCCACTC-3'

pSB1C3_AB_rev (44 bp) 5'-CGATGAAATACGTGGATTAAGCTCGCTTGGACTCCTGTTGATAG-3'

Source

Gibson

References