Part:BBa_K1526000
SpPCS: Phytochelatin synthase from Schizosaccharomyces pombe
The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group in the synthesis of phytochelatin. Overexpression of this gene, together with Gsh1* (BBa_K1526001), in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/).
Gene: spPCS
Organism: Schizosaccharomyces pombe
Function: The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group to synthesise phytochelatins.
Aim: Over-expression of this gene, together with Gsh1*, in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in taking up cadmium.
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 339
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |