Coding

Part:BBa_K1439004:Design

Designed by: Zhaoliang Chen   Group: iGEM14_OUC-China   (2014-10-05)
Revision as of 07:43, 14 October 2014 by Chen.z.l (Talk | contribs) (→‎Design Notes)

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TAT-H4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 260
    Illegal XhoI site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 241


Design Notes

fw-TCCTCGAATTCTTCTAGACATGTATGGTCGGAAAAAACG rv-GTTCTGCAGTACTAGTAATTAGCCGCTGAAGCAGTAG

Source

Histone H4 comes from the Wheat histone H4 gene, and TAT-PTD comes from HIV-1's TAT protein gene.

References