Coding

Part:BBa_K1351027:Design

Designed by: Mona Dotzler   Group: iGEM14_LMU-Munich   (2014-10-05)
Revision as of 10:05, 5 October 2014 by Mdotzler (Talk | contribs)

Signal peptide of B. subtilis minor autolysin LytE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

This part was generated by amplification from BioBrick BBa_K1351007 with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

LytE_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC ATGAAAAAGCAAATCATTACAGCTACGACAGC

LyteSP_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT TGCAGATGCCGCTCCTGCAAATAAC

References

Tjalsma, H., A. Bolhuis, J. D. Jongbloed, S. Bron and J. M. van Dijl. "Signal Peptide-Dependent Protein Transport in Bacillus Subtilis: A Genome-Based Survey of the Secretome." Microbiol Mol Biol Rev 64, no. 3 (2000): 515-47. [http://www.ncbi.nlm.nih.gov/pubmed/10974125 PubMed]