Part:BBa_K1412002
Contents
Basic part of biobrick BBa_K1412001
What it is
Basic part of biobrick K1412001, lacking of promoter BBa_K206000(pTET).
What it does
Through ligating this part with different promoters such as pBAD, pTET, pCONS and transform the plasmid into E.coli(CL-1), we can endow the bacteria the ability of responding to different inducers and inhibitors. On the basis of this mechanism, we could take the E.coli(CL-1) under our command to form different pattern which is mathematically meaning.
How to use it
After ligated with promoter,we could transform this part into CL-1 to obtain the engineering bacteria. Incubate them on the plate with fit concentration of IPTG and chloromycetin, we observed the chemotaxis diameter over time, and got the experimental data. It is also used to form ellipse.
Experimental Data
With K20600(pBAD) as an inhibit promoter
protocol
1.Transformation
2.Extract plasmids
3.Digestion
4.DNA gel electrophoresis
5.Gel Extraction
6.Ligation
7.Transformation
8.Extract plasmids
9.Digestion
10.DNA gel electrophoresis
11.Transform the plasmid into CL-1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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