Part:BBa_K1412002

Basic part of biobrick BBa_K1412001

What it is

Compared to part BBa_1412001, this part substitutes BBa_R0040, a constant expression promoter in our engineered bacteria CL-1, for BBa_K206000.

What it does

As a control group for K1412001, we use this part to eliminate background expression of AraC, and it’s also capable to form ellipse on the plate under the induction of IPTG.

How to use it

Transforming this part into CL-1, we got the engineering bacteria. Incubate them on the plate with fit concentration of IPTG and chloromycetin, we observed the chemotaxis diameter over time, and got the experimental data. It is also used to form ellipse.

protocol

1.Transformation

2.Extract plasmids

3.Digestion

4.DNA gel electrophoresis

5.Gel Extraction

6.Ligation

7.Transformation

8.Extract plasmids

9.Digestion

10.DNA gel electrophoresis

11.Transform the plasmid into CL-1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]