Part:BBa_K1463603

FliC with RBS and J23106 promoter

FliC with B0032 and B0034 RBS under the control of J23106 promoter.

This composite part was made by inserting synthesised oligonucleotides containing J23106 promoter and B0032 into K1463601 (fliC and B0034 RBS).

As shown in Figure 1G and 1H, this construct was able to restore swimming in a fliC knockout strain to near wild-type levels. These results are also shown in Figure 2.

Flic Motility Swarm Assay

Figure 1: FliC Swarm Motility Assays.
(A) DS941, (B) DS941 ΔfliC,
(C) DS941 ΔfliC + pSB1C3 fliC (no promoter), (D) DS941 ΔfliC + J23100 (mutant promoter) fliC,
(E) DS941 ΔfliC + J23116-fliC(1), (F) DS941 ΔfliC + J23116-fliC(2),
(G) DS941 ΔfliC + J23106-fliC(1), (H) DS941 ΔfliC + J23106-fliC(2)

Figure 2 - FliC Motility Histogram

For more information on the biobrick and methods used go to http://2014.igem.org/wiki/index.php?title=Team:Glasgow/Project/Mobility_Proteins#fliC

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1306
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 383
  Illegal AgeI site found at 791
  
• 1000
  COMPATIBLE WITH RFC[1000]