Composite

Part:BBa_K1465209:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-02)
Revision as of 19:43, 3 October 2014 by Tore (Talk | contribs) (→‎Source)

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Carbonic anhydrase (csoS3), shell-proteins (csoS14) and pore protein (csoS1D) of the carboxysome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 166
    Illegal AgeI site found at 2277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the shell-proteins and the carbonic anhydrase (BBa_K1465208) into the pSB1C3 backbone of the pore-forming protein (BBa_K1465207).

Source

BBa_K1465208: shell-proteins (BBa_K1465206) and carbonic anhydrase (BBa_K1465205) from Halothiobacillus neapolitanus.
BBa_K1465207: pore-forming protein csoS1D from Halothiobacillus neapolitanus.

References