Part:BBa_K1470002
Galactose-induced gene 4 DNA binding domain (Gal4DBD)
This protein from Saccharomyces cerevisiae is a positive transcriptional regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which are important for galactose import and conversion to glucose [1].
It recognizes a 17 base pair sequence (5'-CGGN11CCG-3') in the upstream activating sequence (UAS-G) of these genes. [2]
GAL4 is used to investigate gene expressions in several organisms (bacteria, plants, fruit flyes) [3].
The protein is controlled by a strong promotor. Binding to UAS-G leads to the expression and detection of the corresponding gene, GFP for example.
References
[1] TRAVEN, A., JELICIC, B., SOPTA, M.: Yeast Gal4: a transcriptional paradigm revisited. EMBO Rep. May 2006; 7(5): 496–499.
[2] CAMPBELL, R.N., LEVERENTZ, M.L., RYAN, L.A., REECE, R.J.: Metabolic control of transcription: paradigms and lessons from Saccharomyces cerevisiae. Biochem. J. (2008) 414 (177–187).
[3] BRAND, AH., PERRIMON, N.: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993 Jun;118(2):401-15.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 218
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 137
None |