Coding

Part:BBa_K1509000

Designed by: Tong Li   Group: iGEM14_NEFU_China   (2014-09-26)
Revision as of 13:00, 17 October 2014 by LiTong (Talk | contribs)

Coding for the trans-acting regulator SmtB

In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus elongates PCC7942. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn2+ and Cd2+. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn2+ there are four potential metal binding sites on protein SmtB as known as α3, α3N, α5 and α5C [9], different from Zn2+ resistance mechanisms, Cd2+ binds SmtB at the site α3N [10]. These two mechanisms both cause the elevated expression of smtA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 260
    Illegal PstI site found at 349
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 349
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds/transcriptionalregulator/repressor
Parameters
None