Part:BBa_K1509000

Coding for the trans-acting regulator SmtB

In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus sp.. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn2+ and Cd2+. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn2+ due to the classical helix-turn-helix motif similar to many DNA-binding proteins. It can also be genomically rearranged when induced by Cd2+, leading to a deletion within smtB because of a highly iterated palindrome (HIP1), a highly represented octanucleotides(5′-GCGATCGC-3′) which traverse both borders of the excised element. These two mechanisms both cause the elevated expression of smtA.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 349
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 260
  Illegal PstI site found at 349
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 349
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 349
  
• 1000
  COMPATIBLE WITH RFC[1000]