Conjugation
OriTRP4

Part:BBa_K1439000:Experience

Designed by: Wenqi Li   Group: iGEM14_OUC-China   (2014-09-28)
Revision as of 06:23, 29 September 2014 by Tifalee (Talk | contribs) (→‎Applications of BBa_K1439000)


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Applications of BBa_K1439000

We have test the oriT BBa_K1439000 in a conjugation system in which pSB1C3 with help of RP4, and E. coli HB101 as donor and E.coil Top10 as recipient cells. We test the conjugative competence of oriT BBa_K1439000 by conjugate between HB101 and Top10.We use the RFP (BBa_J04450) marks the plasmids containing oriT BBa_K1439000.We get HB101 with out streptomycin resistance form Zhigang Qiu.Top 10 has streptomycin resistance. After conjugation,we select recipient cells by streptomycin resistance culture medium and statistical number of red bacterial colony.

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