Regulatory

Part:BBa_J100176:Experience

Designed by: Jenny Fritz   Group: Campbell M Lab   (2014-09-11)
Revision as of 14:44, 25 September 2014 by Macampbell (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

=Applications of BBa_J100176

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User Reviews

UNIQ3d7eddbe24291833-partinfo-00000000-QINU UNIQ3d7eddbe24291833-partinfo-00000001-QINU

We are testing if triclosan induces the FapR promoter in E.coli cells. This figure shows FapR induction in E. coli by Bacdown hand soap, which contains 0.5% triclosan and surfactants. Because the soap contains surfactants, the bacteria died. The promoter was still able to minimally bind to the cell without the triclosan. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells.