Measurement

Part:BBa_K1330000:Design

Designed by: Rasmus Bech   Group: iGEM14_DTU-Denmark   (2014-09-24)
Revision as of 16:33, 22 October 2014 by KristianJensen (Talk | contribs)

Spinach2.1 flanked by tRNALys3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was synthesised, and amplified using High-Fidelity PCR. The PCR product was cut and ligated with pSB1C3.

The fusion with tRNA was designed as described in (tRNA article). The Spinach2 sequence comes from (Spinach2 article). This BioBrick is a modified version of Spinach2 that has been modified (link to parts page) to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2.


Source

References