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Part:BBa_K1333201:Design

Designed by: Yanwen Xie   Group: iGEM14_SYSU-China   (2014-09-09)
Revision as of 04:19, 24 September 2014 by Chloe Xie (Talk | contribs) (→‎Design Notes)

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lambda cI-LGF2(D78A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The LGF2 is mutated from GAC to GCC at site 78.

Source

It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).

References