Composite

Part:BBa_K1520026

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)
Revision as of 20:07, 12 October 2014 by Sagyman (Talk | contribs)

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Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter. It produces rfp when there is less IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2002
    Illegal NheI site found at 2025
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3203
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
Parameters
biology
controlaTc, tetracyline
directionForward
functionRep
ligandstetracycline
n/aconstitutive promoter family member
negative_regulators1
o_h
o_l
positive_regulators
proteinTetR
swissproP04483
tagLVA