Composite
NemR PRep

Part:BBa_K1398007

Designed by: Exeter iGEM 2014   Group: iGEM14_Exeter   (2014-08-12)
Revision as of 18:14, 2 November 2014 by EdwardMuir (Talk | contribs)

NemR Promoter Reporter

A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008). The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Exeter2014_007_InducableTNTpromoter.jpg

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Categories
Parameters
None