Reporter

Part:BBa_K1333204:Design

Designed by: Yanwen Xie   Group: iGEM14_SYSU-China   (2014-09-09)
Revision as of 13:15, 24 September 2014 by Chloe Xie (Talk | contribs) (→‎Design Notes)


Or2-RBS-GFP-terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 741


Design Notes

This is a composite part including plac Or2-62 and GFP generator. Use a strong RBS and GFP to tell the interactional degree between baits and targets in Bacterial Two-hybrid System.

Source

Mutated LGF2 at site 78 from Asp to Ala and fused it onto lambda cI.

References