Part:BBa_K1442040

MS2 bacteriophage coat protein

The MS2 protein binding box is a highly specific RNA hairpin loop structure in viral RNA that binds the MS2 coat protein of the bacteriophage in order to allow encapsidation of the genome and represses the translational of replicase synthase [1]. Exploiting the MS2 box-protein binding relationship is known as MS2 tagging

Isaacs et al [2] noted that the MS2 protein binding box is of use for engineering RNA transcriptional activators via the DNA-protein binding interactions and the hairpin has thus been shown that the MS2 box can be used to drive expression of proteins when localised to a promoter (e.g. the MS2 protein is fused to a DNA-binding protein and the MS2 box is localised to its target promoter, driving expression).

Furthermore, Auslander et al [3] also showed that the MS2 protein-box relationship can be designed for use in synthetic biology to create programmable single-cell mammalian biocomputers with simple expression logic, increasing the complexity of a informational processing system without researcher input.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 255
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 255
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 255
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 255
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 255
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 292