Coding
REACh1

Part:BBa_K1319001

Designed by: Michael Osthege, Florian Gohr   Group: iGEM14_Aachen   (2014-09-03)
Revision as of 19:36, 3 September 2014 by Mosthege (Talk | contribs) (→‎References)

RFC[25]-compatible dark quencher based on K1319000 (E0030)


This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP).

Two mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W


References

  • Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS. A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP. Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4089-94. Epub 2006 Mar 6. PubMed PMID: 16537489; PubMed Central PMCID: PMC1449651. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449651/?report=classic PubMed Central]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None