Regulatory
FZ
Part:BBa_K1442006
Designed by: Caroline de Cock Group: iGEM14_Warwick (2014-08-14)
Anti-theophylline Aptazyme
An aptazyme is an allosteric ribozyme acting as a regulatory riboswitch. It self-splices in response to theophylline, a drug used to treat asthma and other chronic obstructive pulmonary diseases acting as a bronchodilator. This differs from caffeine by one methyl group and a nitrogen atom creating the structure seen below. This is not endogenous to the body therefore we used this element as a "kill switch" for our system.
One RNA molecule binds to theophylline with a dissociation constant of 0.1microM and Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//biosafety
None |