Coding
E coil fer

Part:BBa_K1189037:Design

Designed by: Chris Wintersinger, Denny Hoang, Taylor Remondini   Group: iGEM13_Calgary   (2013-10-24)
Revision as of 23:30, 31 October 2013 by Cmwinter (Talk | contribs)

His tag + Human ferritin di-subunit with E coil under LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1307


Design notes

This part was created by fusing the heavy chain (BBa_K1189025, P02794 [UniParc]) and light chains (BBa_K1189024, P02792 [UniParc]) of human ferritin together. It is expressed under the LacI promoter (BBa_J04500) and has a his-tag for protein purification. An E-coil (BBa_K1189011) is included in order to allow binding of parts containing the respective K-coil (BBa_K1189010). This particular part is expressed under a strong RBS and the LacI promoter. The iGEM Calgary 2013 team attempted expression and purification of E coil ferritin di-subunit using this system. Additionally, they tested this same coding sequence under the T7 promoter in a different vector.

Source

References