Coding

Part:BBa_K1131006:Experience

Designed by: Ramya Prathuri   Group: iGEM13_Berkeley   (2013-10-28)
Revision as of 05:16, 2 November 2013 by Rprathuri (Talk | contribs) (→‎Applications of BBa_K1131006)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1131006

OleD-K-UDPG-bleck-short.png
Absorbance data obtained from HPLC at 270nm. Reaction containing Kaempferol, OleD, and donor molecule UDP-glucose. Peaks for kaempferol and kaempferol glucoside are observed demonstrating the successful purification of active OleD. HPLC Indican Black 1.png
Absorbance data obtained from HPLC at 270nm. Reaction containing indoxyl, OleD, and donor molecule UDP-glucose peaks for indican and sodium complexed indican are observed along with the precursor indoxyl.
T4 IPO4 UDPG Black 1.png
Mass spectrometry data for the elution fraction at a retention time of 9.51 (Indican peak in HPLC data shown in previous figure). Peaks matching the mass of the precursor indoxyl (134), indican (296), and sodium complexed indican (318) were found. All unlabeled peaks did not correspond to expected ions.
UNIQ69f117d7dedf8bd2-partinfo-00000000-QINU UNIQ69f117d7dedf8bd2-partinfo-00000001-QINU