Coding

Part:BBa_K1111015

Designed by: Sandra Elisabeth Chaudron, Caroline Desmuget and Mareike Apelt   Group: iGEM13_EPF_Lausanne   (2013-10-28)
Revision as of 21:28, 28 October 2013 by Sandelisa90 (Talk | contribs) (→‎Usage and Biology)

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INP_EYFP_Streptavidin Fusion Protein

Introduction

This part is a Fusion protein made respectively of an Ice Nucleation Protein (INP), EYFP and a Streptavidin protein; which is an improved version of BBa_K1111014. INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.

Figure 1: reminder of BBa_K1111014 expected outcome

Usage and Biology

This Biobrick is designed to allow the INP to target streptavidin on the outer membrane of E.coli with EYFP between INP and Streptavidin protein as a reporter.
Indeed EYFP fluorescence will assess of the construct expression and Immunostaining assay (as we did for BBa_K523013) will be used to show the localisation on the cell membrane.
Our final goal is to attach biotynilated Gelatine nanoparticules to the E.coli cells thanks to the biotin-streptavidin interaction.
Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 2426
    Illegal AgeI site found at 2477
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None