Coding

Part:BBa_K1111015

Designed by: Sandra Elisabeth Chaudron, Caroline Desmuget and Mareike Apelt   Group: iGEM13_EPF_Lausanne   (2013-10-28)
Revision as of 21:27, 28 October 2013 by Sandelisa90 (Talk | contribs) (→‎Usage and Biology)

INP_EYFP_Streptavidin Fusion Protein

Introduction

This part is a Fusion protein made respectively of an Ice Nucleation Protein (INP), EYFP and a Streptavidin protein; which is an improved version of BBa_K1111014. INP is an outer membrane protein foud in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.

Figure 1: reminder of BBa_K1111014 expected outcome

Usage and Biology

This Biobrick is designed to allow the INP to target streptavidin on the outer membrane of E.coli with EYFP as a reporter.
Indeed EYFP fluorescence will assess of the construct expression and Immunostaining assay (as we did for BBa_K523013) will be used to show the localisation on the cell membrane.
Our final goal is to attach biotynilated Gelatine nanoparticules to the E.coli cells thanks to the biotin-streptavidin interaction.
Thus, it can be used in any experiment involving biotin-streptavidin to put cargos on bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 2426
    Illegal AgeI site found at 2477
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None